- There are 25 locations for the film, including France, Norway, the Czech Republic and all parts of North America have become battlefields. There are the most well-known landmarks in Paris, and the old streets of the Czech Republic serve as French cities for high-speed chasing scenes in the film. In addition, the cold Norway serves as the arctic scene in the film.
- Originally, the Duke's role planned for Sam Worthington to star, but because of the conflict between the filming of "Special Forces" and "Avatar", Sam Worthington finally turned down the "Special Forces" film.
- Dennis Quaid finished filming all of his scenes in the first two months after filming started.
- In preparation for the role of the baroness, Sienna Miller spent four months on physical training, gaining 5 pounds of muscle, and also learned to shoot live ammunition.
- Channing Tatum originally didn't want to star in this movie because he thought such a movie was glorifying the war. After he watched the script of the film, he thought the film was more like a sci-fi action film so he took the film.
G.I. Joe: The Rise of Cobra behind the scenes gags
G.I. Joe: The Rise of Cobra quotes
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Ripcord: [exercising] Hey, man, Breaker told me Scarlett graduated college at 12 years old. She's like some freaky little deadly genius.
[Ripcord leans on bars]
Duke: Rip. I get it, Rip. You like her.
Ripcord: Sorry, my bad. To hell with it!
[Ripcord lets go]
Duke: Rip. Rip.
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Zartan: Hello, Mr. President. Mr. McCullen sends his regards.
U.S. President: [shocked] Oh, my God. So, that's what this is all about.