In the early days of Japan's Warring States Period, Tokugawa Ieyasu became the ruler of the world, but certain princes of Toyotomi Hideyoshi's faction were ambitious, and some secretly trained assassins, and the former military commander Ohata was the teacher of the assassins. Xiaoba adopted nine men, one woman, and ten orphans, and taught them to become master fighters. Amo was the only girl among them. One day, Xiaoba suddenly gave an order to divide them into five groups to kill each other so that the winner could carry out the task with him. The young people had to die after being shocked, and Amo also personally killed the brother who was in love with him. They realize that as an assassin, killing or being killed is a destiny that must be endured.
After Xiaoban and his party came out of the mountain, they encountered gangsters and bloodbathed the local residents in the small town. He was instructed to kill the three generals around Tokugawa, and the first target was Asano. With his youth and beauty, Amo was able to get close to Asano, who loves fishing, and knocked him out in one fell swoop. The crowd then ambushed the second target, Kato Kiyomasa, but under the full protection of Kato's ninjas headed by the flying monkey, they failed to succeed. Instead, one person was hit by a poisoned sword. After Kato escaped, he began to gather his strength to fight back. First, he found the three brothers Sashiki who took self-mutilation, and then released the bloodthirsty madman beauty pill in prison.
In the small town where they live, there is a quagmire performing Chinese acrobatics. The heroines Yahui and Amo hit it off at first sight, and fell in love with Amo's brother at first sight. The troupe encountered the three Sashiki brothers on the way, and all but Ahui were killed. Amo and others passing by took action and shot the three Sashiki brothers to death. Faced with the poisoned and ill disciple, Ota decided to abandon him. For the first time, Amo and others had a dispute with Master, and even drew their swords. Oba angrily declared that Amo was no longer an assassin, and led others to leave. Xiaoqiao promised Yahui to send her back to her hometown, but when she encountered a beauty pill who was dressed in women's clothing, had a knife in each hand and a rose, the senior was defeated and died. It was too late for Amo to arrive. Yahui asked Amo to walk with her, and for the first time to put on makeup and female clothes for her, Amo seemed to have restored the prosperous nature of a girl. In the night, some robbers grabbed their intent to rape them, and Amo resisted and killed them all. She realized that "killing is her destiny", and only fighting with a knife is her life.
Oba and his disciples assassinated Kato, fell into a siege, wounded and captured. At this moment, Amo rushed to the enemy line of hundreds of people alone, killing Meiwan Maru, but Xiaoban was exasperated. Amo pursued the escaped Kato until he assassinated him at sea, avenging his master and senior brothers
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